Exposing the LASIK Scam

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 Post subject: Drug to induce growth of corneal nerves after LASIK?
PostPosted: Mon Jan 01, 2007 2:06 am 
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Am J Ophthalmol. 2006 Nov 27; [Epub ahead of print]

PACAP Induces Neurite Outgrowth in Cultured Trigeminal Ganglion Cells and Recovery of Corneal Sensitivity After Flap Surgery in Rabbits.

Fukiage C, Nakajima TA, Takayama YO, Minagawa YO, Shearer TH, Azuma MI.
Senju Laboratory of Ocular Sciences, Senju Pharmaceutical Co Ltd, Kobe, Hyogo, Japan.

PURPOSE: To evaluate the ability of pituitary adenylate cyclase-activating polypeptide (PACAP) to induce growth of neuronal processes in cultured trigeminal ganglion cells, and to accelerate neurite outgrowth and recovery of corneal sensitivity after creation of a corneal flap in a rabbit model of laser-assisted in situ keratomileusis (LASIK) surgery.

DESIGN: Animal study.

METHODS: The cDNA of rabbit PACAP was sequenced, and the expression of PACAP receptors in the trigeminal ganglia from rabbits was quantified by quantitative real-time polymerase chain reaction. Trigeminal ganglion cells were isolated from rabbits and cultured for 48 hours with or without PACAP27 (bioactive N-terminal peptide from PACAP). Cells were stained with antibody against neurofilaments, and neurite outgrowth was quantified by cell counting. In the rabbit LASIK model, a corneal flap with a planned thickness of 130 mum and 8.5 mm diameter was created with a microkeratome. The rabbits then received eyedrops containing PACAP27 four times a day for eight weeks, and corneal sensitivity was measured. Neurite outgrowth was assessed by staining histologic sections of the flap area for cholinesterase.

RESULTS: The deduced amino acid sequence of PACAP in rabbit was identical to that of human. PACAP receptor, PAC1, was highly expressed in trigeminal ganglia from newborn and adult rabbits. PACAP27 at 1 muM induced growth of neuronal processes in cultured primary trigeminal ganglion cells. In the LASIK model, extensions of neuronal processes from amputated nerve trunks in cornea were observed after administration of eyedrops containing 1 or 10 muM PACAP27. The 10 muM PACAP27 treatment also greatly accelerated recovery of corneal sensitivity.

CONCLUSIONS: PACAP may be a candidate drug for ameliorating dry eye after LASIK surgery.

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 Post subject:
PostPosted: Fri May 18, 2007 3:27 am 
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Joined: Sat May 12, 2007 9:18 pm
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Location: Studio City, CA
Why would a science lab try to stimulate growth of corneal nerve endings?
Unless there's a need for such a thing.
Someone should write them a thank-you note. To let them know that there are people who need such treatment. I wonder if I have to learn Japanese.


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 Post subject: Stimulating nerve growth
PostPosted: Sat Jun 30, 2007 5:57 pm 
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There is a great deal of interest in stimulating nerve growth/regrowth to help patients who have nerve damage in other parts of the body. There is not only fame, but big money in store for the lab that is able to solve the mysteries of nerve regeneration. Some amphibians do it well - we can't! Why not? It's a mystery.

Fixing the nerve regrowth problem won't make LASIK a safe and effective surgery, but it may provide relief some day to some patients who have nerve damage from corneal refractive surgery. That would merit a nice thank-you note - but I'm saving my really big thank-you note for the folks who help stop LASIK altogether. :wink:

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We can easily forgive a child who is afraid of the dark. The real tragedy of life is when men are afraid of the light. -Plato


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